The Miller Apparatus 245


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ists knew that the Law of Mass Action would almost immediately have destroyed the amino acids that were formed, without a trap to catch them in quickly. The "primitive ocean" must have had similar bottle traps in it.)

After a week of this, the fluid in the traps were chemically analyzed—and were found to have microscopic traces of a few L and D (right- and left-handed) nitrogen-containing com-pounds—"amino acids," they called them—which had been formed. (Of course, if both L and D amino acids were formed by chemical action—as they always are when formed outside of living cells—it would be impossible for the amino acid which formed to be useable for life purposes.)

Newspapers around the world heralded the news: "Life has been created!" But no life had been created, just a few biochemical compounds. Remember that neither nitrogen compounds nor amino acids are, of themselves, living things. Just because they are in living things, does not make them living things.

In summary then, *Stanley Miller's experiment was one of the early origin-of-life attempts. It used a reducing atmosphere (with no oxygen in it). A significant part of his experiment was a "cold trap." This was a glass cup at the bottom of the tubing that caught the products of the week-long water-chemical-spark activity. The purpose of the trap was to keep the reaction going in the right direction. If it had not been there, the simple amino acids would have been destroyed faster than they could be made!

" 'This is the primitive atmosphere,' said Stanley Miller, the chemistry professor at the University of California at San Diego, as he pointed to the transparent mixture of gases inside the globe. 'And this represents the primitive ocean,' he said, indicating a pool of water in the bottom of his apparatus."—*Rick Gore, "Awesome Worlds Within a Cell, "National Geographic Society, September 1976, p. 390.

What does that complicated lab experiment have to say about the possibility of nature doing it by accident— without the help of man? Outdoors, it could not be done without his help—or with it.

"What we ask is to synthesize organic molecules without such a machine. I believe this to be the most stubborn problem that confronts us—the weakest link at present in our argument."—*G. Wald, "The Origin of Life, " in the Physics and Chemistry of Life (1955), p. 9.

The test tube attempts to "create life" have only resulted in dismal failure.

"In 1953, at the University of Chicago, Stanley L. Miller and Harold C. Urey mixed ammonia, water vapor, hydrogen and methane to simulate Earth's early atmosphere, then crackled lightning-like electrical sparks through it . .

"Unfortunately, as Margolis admits, 'no cell has yet crawled out of a test tube,' and thousands of similar experiments have produced goopy organic tars, but no recognizable life. Decades of persistent failure to 'create life' by the 'spark in the soup' method (or to find such productions in nature) have caused some researchers to seek other approaches to the great enigma . . [He then discussed panspermia theories: the possibility of bacteria flying in from outer space.]"—*RichardMilner, Encyclopedia of Evolution (1990), p. 274.

NOT LEFT-HANDED AMINO ACIDS—Every type of protein in animals is left-handed (L-aminos). None are ever right-handed (D-aminos). Yet all amino acids synthesized in laboratories consist of an equal amount of left- and right-handed amino acids (a racemic mixture). It would require days of work in the laboratory to separate just a few L from D forms. Researchers cannot figure out how to produce only the L form. Yet no animals or man could live if they had any of the D form in them. This is a major problem to the evolutionists. More on this in the next chapter.

NOT THE ESSENTIALAMINO ACIDS—Out of the hundreds of possible combinations, there are 20 essential amino acids, yet laboratory synthesis of amino acids produces only a few of the 20 essential amino acids—plus a lot of non-essential or even useless ones.

THE OPARIN EXPERIMENT—Prior to *Miller, *A.I. Oparin, a Russian chemist, tried to produce living cells from coacervates, which are like fat droplets in a bowl of soup. He carefully kept all oxygen away from the soup and the bowl, and he hoped that, given enough time, they would join together and, somehow, life would enter into them! But the outer film kept breaking apart, and no life entered into them. *Oparin was disappointed. No reputable chemist today considers Oparin's theory to be of any value.

THE FOX EXPERIMENTS—After *Miller's experiment, * Sydney Fox in 1960 worked out a different arrangement, but he began his with left-handed amino acids already formed. He took them from a dead animal! He claims that his method is how it was done in the primitive environment. This should have been good news for the evolutionary world; but, when we learn his complicated procedure, we can understand why few scientists have any faith in the possibility that the Fox procedure was done by chance in the ocean, near a volcano, or in a mud puddle.

Here is how nature, armed with time and chance, is supposed to have produced that first dead amino acid: "Typical panpolymenzation: Ten grams of L. glutamic acid (a left-handed amino acid] was heated at l75o-l80o C. [347°-356° F.) until molten (about 30 minutes), after which period it had been largely converted to lactum. At this time, 10 g. [.352 ay. oz.] of DL-aspartic acid and 5 g. [.176 ay. oz.] of the mixture of the sixteen basic and neutral (BN) amino acids were added. The solution was then maintained at 170° + or -2° under an atmosphere of nitrogen for varying periods of time. Within a period of a few hours considerable gas had been evolved, and the color of the liquid changed to amber. The vitreous mixture was rubbed vigorously with 75 ml. [4.575 Cu. in.] of water, which converted it to a yellow-brown granular precipitate. After overnight standing, the solid was separated by filtration. This was washed with 50 ml. [3.05 cu. in.] of ethanol, and as substance S dialytically washed in moving Multidialyzers in water for 4 days, the water being changed thrice daily. (The term dialytic washing indicates dialytic treatment of a suspension.) In some preparations, the solid was dissolved completely in sodium bicarbonate solution and then dialyzed. The dialysis sacs were made of cellulose tubing, 27/32 in., to contain 50 ml. [3.05 cu. in.]. The nondiffusible material was ninhydrin-negative before the fourth day. The non-aqueous contents of the dialysis sac were mainly solid A and a soluble fraction B recovered as solid by concentration in a vacuum dissicator. The mother liquor of S was also dialyzed for 4 days, and then dried to give additional solid C."—*S.W. Fox and *K. Harada, Journal of the American Chemical Society, 82(1960), p. 3745.

We commend *Sydney Fox and his associates for their remarkable intelligence and excellent lab equipment, days of exhausting work, and the university scientists who trained them to perform such experiments. But we can make no such commendation of sand, gravel, and seawater, which is supposed to have done the same thing by itself.

Fox began with a quantity of left-only (no right) amino acids and made sure no oxygen, sugars, etc. were present, since they would doom the experiment. Then he underwent a lot of tedious work that requires a high degree of intelligence, careful planning, and many adjustments with pH, temperature, cooking time, etc. as he proceeded with a staff of assistants.

Fox is modest about his abilities, for he says that random events, in a broad sea or on the slopes of a volcano, could have done it just as easily. But HE began with pure, left-handed amino acids, which are available nowhere outside of living things; he did not begin with pebbles, mud, and water.

Fox then heated the amino acids for 10 hours at 150°-180° C [302°-356°] for several hours. Pretty hot way to make amino acids!

Where would you find such conditions in nature? * Stanley Miller, who first synthesized amino acids in a laboratory later stated that his own experiment could not possibly have been done by chance outside of a modern laboratory. Other scientists have agreed.

"Such experiments are no more than exercises in organic chemistry."—*P. Mora, "The Folly Of Probability, " in Origins of Prebiological Systems and their Molecular Matrices, Ed. *S. W. Fox (1965), p. 41.

Three key ingredients are (1) proper chemicals in exacting amounts, (2) a continuous energy source (such as a continuous spark), and (3) quick-dry apparatus. As soon as the amino acids are made, they must immediately be dried out. (Living tissue never contains dried out amino acids or comes from it.) Fox tells us the reaction must be "hot and dry" (op. cit., p. 378).

"To keep a reaction going according to the law of mass action, there must be a continuous supply of energy and of selected matter (molecules) and a continuous process of elimination of the reaction products."—Op. cit., p. 43.

And there is a fourth key ingredient: Whether done in nature, or by researchers in a high-tech laboratory, these life substances are always the result of careful organization with specific _purposes by a high-level intelligence. No one tosses the chemicals into a pan in the laboratory, walks off, hoping it will produce amino acids all by itself.

A living organism is not just dried out ocean soup. It is highly integrated, complex, and purposive. —It has life, which no man can produce. And that living creature had to have all its parts on Day One of its existence. And it had to have a mate and be able to reproduce offspring.

Not even *Darwin could figure it out.

"Darwin never really did discuss the origin of species in his [book] On the Origin of Species."—*David Kitts, "Paleontology and Evolutionary Theory, " Evolution,

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